BIO 339M & 393

Spring 2009

Dr. Rasika Harshey

TTh 2 - 3.30 PM, RLM 7.114

Office Hours:

By appointment at NMS 2.118

Phone Number:

471-6881

Email:

rasika@uts.cc.utexas.edu

 

*  Syllabus                 Groups

You are responsible for printing online articles. I suggest you either download the PDFs on your laptops or e-mail them to yourself while on campus, since you may not be able to access many of the links from elsewhere.

 
* Topics

Jan 20            Introduction

Jan 22            Overview of chemotaxis 

Jan 27            Discovery of chemoreceptors

Jan 29         Discovery of flagellar rotation      (Group 1)

Feb 3             Gradient sensing and chemotaxis     (Group 2)

Feb 5             Transmembrane signaling in a chemoreceptor    (Group 3)        

Feb 10            Signaling through a dimer    (Group 4)      

Feb 12           Cytoplasmic signaling pathway: CheA      (Group 5)   

Feb 17            Regulation by phosphorylation: CheB       (Group 6)

Feb 19            Regulation by phosphorylation: CheY    (Group 7)   

Feb 24             Review     

Feb 26             Test 1      Key   

Mar 3               Chemoreceptor clustering                     

Mar 5               Trimer-of-dimers                                           

Mar 10             Clustering & Adaptation       

Mar 12             Morphogenetic signals: Flagella assembly   

 

SPRING BREAK

 

Mar 24            Morphogenetic signals: Needle length control   

Mar 26             Surface (?) signals: Swarming in Vibrio                                           

Mar 31                                           :Swarming in Salmonella 

Apr 2                                              :Twitching in Pseudomonas /Myxococcus                            

Apr 7                Review                                                      

Apr 9                Test 2      Key           

Apr 14              Signaling for gene expression    

Apr 16              Cell density signals: Quorum sensing  (Social Cheating) - Devis Bhakta

Apr 21                                              :Quorum sensing (sRNA) - Jong-Han Ho

Apr 23                                               :Biofilms and quorum sensing   - Tony Urbina

Apr 28                                               :Biofilms and motility - Ates Haner

Apr 30            c-di-GMP signals: Caulobacter development  - Abel Flores

May 5              Antibiotics and death signals - Yilie Gu

May 7             Test 3  Key

*   Reading

Jan 22- Feb 19

Mar 3-Apr 2

 

Apr 14

Makino et al., 1996. DNA binding of PhoB and its interaction with RNA polymerase. J. Mol.Biol. 259:15-26. 

 

Apr 16

Review: Henke JM and Bassler BL. Bacterial social engagements. Trends Cell Biol. 2004 14 :648-656.

 

Sandoz et al 2007. Social cheating in Pseudomonas aeruginosa quorum sensing. Proc Natl Acad Sci U S A. 104:15876-15881

 

Apr 21

1. Lenz et al., 2004. The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae. Cell 118:69-82

 

Apr 23

1. Mini-review: Kolter & Greenberg.  2006. Microbial sciences: the superficial life of microbes. Nature. 441:300-302.

2. Davies et al. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science. 280: 295-298.

 

Apr 28

1. Commentary. Berry and Armitage, 2008. How bacteria change gear. Science 320, 1599-1600.

2. Blair et al. 2008 A molecular clutch disables flagella in the Bacillus subtilis biofilm. Science 320, 1636-2638.

 

Apr 30

Paul et al. 2004. Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain. Genes Dev. 18:715-27.

 

May 5

1. Novak et al. 1999. Emergence of vancomycin tolerance in Streptococcus pneumoniae. Nature 399: 590-593.

2. Novak et al. 2000. Signal transduction by a death signal peptide. Mol. Cell 5: 49-57.

 

 

      

 
 

 

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