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Animations  

DNA Replication

  
bullet Helicase
 (CBI CD, 05-1)
bullet Replication 1
 (Lodish 4th ed. CD 12-01)
bullet Replication 2
 (Lodish 4th ed. CD 12-02)
bullet Replication 3
 (Lodish 4th ed. CD 12-03)
bullet Replication 4
 (Lodish 4th ed. CD, 12-04)
   

Translations

  
bullet EF-Tu
 (CBI CD, 03-10)
bullet mRNA
 (CBI CD, 06-4)
bullet Elongation
 (CBI CD, 06-5)
bullet Ribosome Ratchet
 (CBI CD, 06-6)

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Handouts  
Introduction  
bullet Application of Koch's Postulates
(Tortora, 7th ed. Figure 14.3)
   
Staining  
bullet The Gram Staining Procedure
 (Prescott, Figure 2.14)
   
Prokaryotic Cell Structure  
bullet Chemical Composition of a Prokaryotic Cell
 (Brock–Madigan, Table 3.2)
bullet The Location of Macromolecules in the Cell
 (Brock–Madigan, Figure 3.3)
   
Plasma Membrane  
bullet Some Functions of Prokaryotic and Eukaryotic Cell Membranes and Organelles
Microbe by Schaechter et.al.
bullet The Structure of a Polar Membrane Lipid
 (Prescott, Figure 3.5)
bullet Plasma Membrane Structure
 (Prescott, Figure 3.7)
bullet Archael Membrane Lipids
 (Prescott, Figure 20.3)
bullet Examples of Archael Membranes
 (Prescott, Figure 20.5)
bullet ABC Transporter Function
 (Prescott, Figure 5.3)
bullet Active Transport Using Proton & Sodium Gradients
 (Prescott, Figure 5.4)
bullet Group Translocation: Bacterial PTS Transport
 (Prescott, Figure 5.5)
   
Cell Wall  
bullet Cell Wall Structure
 (Ingraham, Page 77)
bullet Peptidoglycan Sub-unit Composition
 (Prescott, Figure 3.16)
bullet The Structure of Pseudomurein
 (Prescott, Figure 20.2)
bullet Diaminoacids Present in Peptidoglycan
 (Prescott, Figure 3.17)
bullet Peptidoglycaan Cross-links
 (Prescott, Figure 3.18)
bullet Peptidoglycan Structure
 (Prescott, Figure 3.19)
bullet The Gram-Positive Envelope
 (Prescott, Figure 3.21)
bullet Teichoic Acid Structure
 (Prescott, Figure 3.22)
bullet The Gram-Negative Envelope
 (Prescott, Figure 3.23)
bullet Lipopolysaccharide Structure
 (Prescott, Figure 3.25)
bullet Protoplast Formation
 (Prescott, Figure 3.26)
bullet Peptidoglycan Synthesis
 (Prescott, Figure 10.28)
bullet Transpeptidation
 (Prescott, Figure 10.29)
bullet Wall Synthesis Patterns
 (Prescott, Figure 10.30)
   
Protein Secretion In Procaryotes  
bullet Protein Secretion Systems
 (Prescott, Figure 3.27)
   
Flagella  
bullet The Ultrastructure of Bacterial Flagella
 (Prescott, Figure 3.33)
bullet Growth of Flagellar Filaments
 (Prescott, Figure 3.34)
bullet Flagellar Motility
 (Prescott, Figure 3.35)
bullet Flagella Biosynthesis
 (Brock-Madigan, Figure 4.42)
bullet Mechanism of Flagellar Movement
 (Prescott, Figure 3.36)
   
Endospore  
bullet Sterilization of Broth Media by Tyndallization
  
bullet Examples of Endospore Location and Size
 (Prescott, Figure 3.40)
bullet Endospore Structure
 (Prescott, Figure 3.41)
bullet Endospore Formation: Life Cycle of Bacillus Megaterium
 (Prescott, Figure 3.43)
bullet  Endspore Germination
 (Prescott, Figure 3.44)
bullet Differences Between Endospores and Vegetative Cells
 (Brock_Madigan, Table 4.2)
   
Growth and Control of Microorganisms  
bullet Microbial Responses to Environmental Factors
 (Presscott Figure 6.3)
bullet Effect of Temperature on Growth Rate
 (Brock-Madigan Figure 6.16)
bullet Oxygen and Bacterial Growth
 (Prescott Figure 6.14)
bullet Physical Methods Used to Control Microbial Growth
 (Tortora Table 7.5)
bullet Chemical Agents Used to Control Microbial Growth
 (Tortora Table 7.8)
bullet Sterilization of Broth Media by Tyndallization
  
   
Energy and Metabolism  
bullet High-energy Bonds
 (Brock Figure 5.12)
bullet Three Stages of Catabolism
 (Prescott Figure 9.3)
bullet Regeneration of NAD During Fermentation
 (Prescott Figure 9.9)
bullet Calvin Cycle (Photosynthesis)
 (Prescott Figure 10.4)
bullet Gluconeogenesis
 (Prescott Figure 10.5)
bullet Organization of Anabolism
 (Prescott Figure 10.17)
   
DNA Replication  
bullet DNA Replication
  
bullet DNA replication and cell division in a prokaryote
(Lodish Figure 12-6)
bullet Consensus sequence of the minimal bacterial replication origin based on analyses of genomes from six bacterial species.
(Lodish Figure 12-5)
bullet Model of Initiation of Replication at E.coli ori C
(Lodish Figure 12-7)
bullet The DNA Polymerase Reaction and Its Mechanism
 (Prescott Figure 11.14)
bullet Bacterial DNA Replication
 (Prescott Figure 11.15)
bullet The DNA Ligase Reaction
 (Prescott Figure 11.17)
bullet Properties of DNA Polymerases
(Lodish Table 12-1)
bullet A Hypothetical Model for Activity at the Replication Fork
 (Prescott Figure 11.16)
bullet Schematic model of the relationship between E. coli replication proteins at a growing fork
(Lodish Figure 12-11)
bullet Mechanism of Action of Telomerase
(Lodish Figure 12-13)
bullet E. coli DNA polymerase III
(Lodish 4th edition, pages 462-464)
   
Transcription  
bullet The Basic Mechanism of Transcription of DNA into RNA
(Lodish Figure 4-15)
bullet Schematic representation of the major form of E. coli RNA polymerase bound to DNA
(Lodish Figure 10-10)
bullet Transcription of Bacterial Gene
(Albert Figure 7-9)
bullet Direction of Transcription
(Albert Figure 7-10)
bullet Sigma Factors of E. coli
(Lodish Table 10-1)
bullet Promoters recognized by E. coli RNA polymerase containing sigma 70.
(Lodish Figure 10-11)
bullet Sigma Factors and Promoter Recognition in Bacteria
 (New Science Press Ltd. 2002)
   
Translation  
bullet Structure of Typical Prokaryotic mRNA
(Albert Figure 7-29)
bullet Polyribosome
(Albert Figure 7-31)
bullet tRNA Synthetase
(Albert Figure 7-23)
bullet Initiation of Protein Synthesis
(Prescott Figure 12-13)
bullet Initiation of Protein Synthesis
(Prescott Figure 12-14)
bullet Elongation of Protein Synthesis
(Prescott Figure 12-15a)
bullet Elongation of Protein Synthesis
(Prescott Figure 12-15b)
bullet Transpeptidation
(Prescott Figure 12-16)
bullet Termination
(Prescott Figure 12-17)
bullet Role of tmRNA
 (Alberts, Fig. 6-76, 1 & 2)
bullet Chaperone & Protein Folding
(Prescott Figure 12-18)
bullet Protein Splicing
(Prescott Figure 12-20)
   
Gene Regulation  
bullet Overview
 (Brock Figure 8-1)
bullet Overview
 (Brock Figure 8-2)
bullet Overview
 (Brock Figure 8-3)
bullet Overview
 (Brock Figure 8-4)
bullet DNA Binding Proteins
 (Brock Figure 8-7)
bullet Enzyme Repression
 (Brock Figure 8-10)
bullet Enzyme Induction
 (Brock Figure 8-11)
bullet Repression and Induction
 (Brock Figure 8-13)
bullet Repression and Induction
 (Brock Figure 8-14)
bullet Repression and Induction
 (Brock Figure 8-16)
bullet Dual Control of the lac Operon
 (Alberts Figure 9-29)
bullet Attenuation Structure of the Tryptophan operon
 (Brock Figure 8-20)
bullet Attenuation
 (Brock Figure 8-21)
bullet Attenuation
 (Lecture Slide 46)
bullet Attenuation
 (Lecture Slide 47)
bullet Attenuation
 (Lecture Slide 48)
bullet Attenuation
 (Lecture Slide 49)
bullet Regulation by sigma factor:
 (Prescott Fig 12.32)
bullet Sigma Factors and Promoter Recognition in Bacteria
 (NSP Ltd. 2002)
bullet Regulation by antisense RNA
 (BenjaminLewin Figure 10.44)
bullet Chemotaxis Text
  
bullet Chemotaxis Image
  
bullet Intermolecular signaling between chemosensory receptors and flagellum in bacterial Chemotaxis (Encyclopedia of Molecular Biology Figure B6)
  
bullet Transducer states in the cycle of excitation and adaptation of the chemosensory receptors in E. coli. (Encyclopedia of Molecular Biology Figure B4)
  
bullet Control of cell cycle
(Prescott Fig 12.34)
bullet Control of cell cycle
(Prescott Fig 12.35)
   
Mutant Isolation and Selection  
bullet Wobble Base Pairing
(Lodish Fig 4-27)
bullet Mutations and Their Chemical Basis
  
bullet Mutations and Mutagenesis
  
bullet Other Types of Mutations
  
bullet How Mutations Arise
  
bullet Spontaneous Mutations
  
bullet Detection and Isolation of Mutants
  
bullet Mutant Detection
  
bullet Treatment of E. coli Cells With a Mutagen
(Prescott Fig 11.30)
bullet Mutant Selection
  
bullet Penicillin Selection
  
bullet Carcinogenicity Testing
  
bullet Comparison of Reversion Rates With and Without Carcinogen
(Prescott Fig 11.32)
bullet In Vitro Mutagenesis
  
bullet Site Directed Mutagenesis
 (Molecular Biology of the Cell, 4th ed.)
   
DNA Repair  
bullet The Expanding Polymerase Universe (Nature Reviews Vol 1 Nov 2000 pg 101)
  
bullet DNA Repair Mechanisms (Snustad, Simmons pp.383-387)
  
bullet SOS Mutagenesis (Nature Reviews Vol 1 Nov 2000 pg 105)
  
bullet SOS Repair
(Prescott Fig 11.34)
   
Bacterial Genetics  
bullet General Recombination
(Prescott Fig 13.2)
bullet General Recombination (cotd)
(Prescott Fig 13.2 cotd)
bullet A simplified version of one molecular mechanism of genetic recombination
(Brock Fig 10.9)
bullet Nonreciprocal General Recombination
(Prescott Fig 13.3)
bullet Gene Transfer
(Brock Fig 10.11)
bullet Detection of Recombinants
(Brock Fig 10.10)
bullet Complementation Analysis
(Brock Fig 10.27)
bullet Fate of DNA
(Prescott Fig 13.4)
bullet Fertility Factors
(Prescott Fig 13.5)
bullet F-factor Integration
(Prescott Fig 13.7)
   
Transposable Elements  
bullet Transposon
(Prescott Fig 13.8)
bullet Transposition 1
(Prescott Fig 13.9)
bullet Transposition 2
(Prescott Fig 13.10)
bullet Transposition 3
(Prescott Fig 13.10 cotd)
   
Bacterial Conjugation  
bullet Bacterial Conjugation
(Prescott Fig 13.12)
bullet U-tube Experiment
(Prescott Fig 13.13)
bullet F+ X F- Conjugation
(Prescott Fig 13.14a)
bullet Hfr X F- Conjugation
(Prescott Fig 13.14b)
bullet F` X F- Conjugation
(Prescott Fig 13.15a)
bullet F` X F- Conjugation (cotd)
(Prescott Fig 13.15b)
bullet Interrupted Mating
(Prescott Fig 13.22a)
bullet Interrupted Mating (cotd)
(Prescott Fig 13.22b)
   
Transformation  
bullet Transformation with DNA Fragments
(Prescott Fig 13.16a)
bullet Transformation with a Plasmid
(Prescott Fig 13.16b)
bullet Mechanism of Transformation
(Prescott Fig 13.17)
   
Transduction  
bullet Phage Lambda Life Cycle
(Prescott Fig 13.18)
bullet Generalized Transduction
(Prescott Fig 13.19)
bullet Specialized Transduction
(Prescott Fig 13.20a)
bullet Specialized Transcution (cotd)
(Prescott Fig 13.20b)
bullet Mechanism of Transduction
(Prescott Fig 13.21a)
bullet Mechanism of Transduction (cotd)
(Prescott Fig 13.21b)
   
Severe Acute Respiritory Syndrome (SARS)  
bullet Coronavirus Linked to SARS: WHO
 
   
Sequencing  
bullet Whole Genome Sequencing
(Prescott Fig 15.3)
bullet Sanger's Sequencing (Enzymatic Method)
(Albert Fig 10.5)
bullet Polymerase Chain Reaction
(Lodish Fig 7.38)
   

 

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