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Waris Medium  

Directions 
Modification of the original recipe. Suitable for xenic cultures of some desmids.

For 1 L Total
1. To approximately 900 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Adjust the pH to 6.0.
3. Bring the total volume to 1 L with dH2O.
4. Cover and autoclave medium.
  
#ComponentAmountStock Solution
Concentration		Final Concentration
1KNO3 (Baker 3190)1 mL/L10 g/100 mL dH200.99 mM
2MgSO4·7H2O (Sigma 230391)1 mL/L2 g/100 mL dH200.081 mM
3(NH4)2HPO4 (Fisher A686)1 mL/L2 g/100 mL dH200.151 mM
4CaSO4.2H2O (Mallinckroft 4300)30 mL/L0.2 g/100 mL dH200.35 mM
5P-IV Metal Solution6 mL/L  
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