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Soilwater: VT Medium  

Directions 
Soil having little or no organic content is substituted for garden-type soil; lacks supplements; for some strains of Closterium, freshwater dinoflagellates and others.

For 200 mL Total
1. Combine components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day.
3. Refrigerate 24 hours or more and bring to room temperature before use.
  
#ComponentAmountStock Solution
Concentration		Final Concentration
1Vermont Soil5 mL/200 mL of dH20  
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