For 1 L Total
1. Prepare 1 L of Waris Medium, but do not adjust the pH.
2. Prepare 200 mL of Euglena Medium.
3. Discard 200 mL of the prepared Waris Medium. Transfer the remaining 800 mL to a 2 L Erlenmeyer flask.
4. Transfer 200 mL of the previously prepared Euglena Medium into the flask.
* For agar add 10.0 g of agar into the flask; do not mix.
5. Cover and autoclave.
6. Store at refrigerator temperature.