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Volvox-Dextrose Medium  

Directions 
For 1 L Total
1. Prepare 1 L of Volvox Medium, except add 10 g of glucose after all other stock solutions have been added.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Volvox Medium1 L  
2Glucose
(BACTO-dextrose or Sigma D 9634)
10 g/L 0.06 M
3Vitamin B121 mL/L  
4Biotin Vitamin Solution1 mL/L  

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