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Polytomella Medium  

Directions 
Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

For 1 L Total
1. To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O .
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Yeast extract
(Bacto, Difco)
1 g/L  
2Tryptone (Sigma T 9410))1 g/L  
3Sodium acetate (Fisher BP 333)2 g/L 24.4 mM

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