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P49 Medium

Directions

Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium.

For 1 L Total
1. To approximately 900 mL of dH₂O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Adjust the pH to 7.0.
3. Bring the total volume to 1 L with dH₂O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
6. Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
2	MgSO₄·7H₂O (Sigma 230391)	1 mL/L	4 g/100 mL dH20	0.16 mM
3	Na₂glycerophosphate^.5H₂O (Sigma G 6501 )	1 mL/L	5 g/100 mL dH20	0.16 mM
4	KCl (Fisher P 217)	1 mL/L	5 g/100 mL dH20	0.67 mM
5	Glycylglycine (Sigma G 1002)	10 mL/L	5 g/100 mL dH20	3.8 mM
6	P-IV Metal Solution	6 mL/L
7	NH₄NO₃ (Fisher A676)	10 mL/L	1 g/100 mL dH20	1.2 mM
8	CaCl₂·2H₂O (Sigma C-3881)	10 mL/L	0.74 g/100 mL dH20	0.5 mM
9	Yeast extract (Bacto, Difco)	0.2 g/L
10	Tryptone (Sigma T 9410))	0.4 g/L
11	Vitamin B₁₂	1 mL/L
12	Biotin Vitamin Solution	1 mL/L
13	Thiamine Vitamin Solution	1 mL/L

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