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1% F/2

Directions

For 1 L Total
1. Measure 285 mL of non-pasteurized seawater (30-35 ppt).
2. Add 600 mL of dH₂O to bring the volume to approximately 900 mL.
3. Add each of the components in the order specified (except vitamins) while stirring continuously.
4. Bring up to 1 L total volume with Pasturized Seawater.
*For 1.5% agar medium: add 15 g of agar into the flask; do not mix.
5. Cover and autoclave medium.
6. Add sterile vitamins to cooled medium.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
7. Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Seawater (non-sterilized)	285 mL
2	dH₂O	600 mL
3	NaNO₃ (Fisher BP360-500)	1 mL/L	7.5 g/100 mL dH20	880 µM
4	NaH₂PO₄·H₂O(MCIB 742)	1 mL/L	0.5 g/100 mL dH20	36 µM
5	Na₂SiO₃·9H₂O (Sigma 307815)	1 mL/L	2 g/100 mL dH20	70 µM
6	Trace Metals Solution	1 mL/L
7	Vitamin B₁₂	1 mL/L
8	Biotin Vitamin Solution	1 mL/L
9	Thiamine Vitamin Solution	1 mL/L

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