UTEX The Culture Collection of Algae

Header

Media Detail

Home

Culture Search

Culture Maintenance

Ordering

Media

Select Collections

Depositing Strains

Taxonomic Considerations

Cryopreservation of Microalgae

Resources

FAQ

N/20 Medium

Directions

For 1 L Total
1. To approximately 850 mL of dH₂O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Bring the total volume to 1 L with dH₂O.
3. Cover and autoclave medium.
4. When cooled add vitamins.
5. Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	KH₂PO₄ (Sigma P 0662)	2 mL/L	17.5 g/200 mL dH2O	1.29 mM
2	NaNO₃ (Fisher BP360-500)	100 µL/L	25 g/200 mL dH2O	0.14 mM
3	K₂HPO₄ (Sigma P 3786)	1 mL/L	15 g/200 mL dH2O	0.43 mM
4	NaCl (Fisher S271-500)	1 mL/L	5 g/200 mL dH2O	0.42 mM
5	CaCl₂·2H₂O (Sigma C-3881)	1 mL/L	5 g/200 mL dH2O	0.17 mM
6	MgSO₄·7H₂O (Sigma 230391)	1 mL/L	15 g/200 mL dH2O	0.3 mM
7	P-IV Metal Solution	6 mL/L
8	Sodium acetate (Fisher BP 333)	0.67 g/L		8.11 mM
9	TES buffer (Sigma T 1375)	0.92 g/L		4.01 mM
10	Vitamin B₁₂	1 mL/L
11	Thiamine Vitamin Solution	1 mL/L

footer

About UTEX \| Contact UTEX

The National Science Foundation	The University of Texas at Austin	Revenue from Cultures