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Soilwater: GR+/NH4 Medium  

Directions 
The basic garden-type soilwater; includes a pinch of CaCO3, which is added to the soil and water after steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add Vitamin B12 to cultures of Volvox; a pinch of NH4MgPO4. 6H2O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed.

For 200 mL Total
1. Prepare GR+ Medium.
2. Add pinch of NH4MgPO4 after pasteurizing.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Soilwater: GR+ Medium200 mL  
2NH4MgPO4 (Sigma 529354)1 mg/200 mL dH2O 0.04 mM

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