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Soilwater: GR- Medium  

Directions 
A garden-type soilwater without supplements. Suitable for Spirogyra, Zygnema and a few other phototrophic algae.

For 200 mL Total (pH 7.3)
1. Combine components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
3. Refrigerate 24 hours or more and bring to room temperature before use.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Green House Soil5 mL/200 mL of dH20  
2dH2O200 mL  

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