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Euglena Medium  

Directions 
Suitable for most axenic cultures of Euglena and Chlorogonium.

For 1 L Total
1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.
  
#ComponentAmountStock Solution
Concentration		Final Concentration
1Sodium acetate (Fisher BP 333)1 g/L 12.2 mM
2Beef extract
(Sigma B-4888)		1 g/L  
3Tryptone (Sigma T 9410))2 g/L  
4Yeast extract
(Bacto, Difco)		2 g/L  
5CaCl2·2H2O (Sigma C-3881)0.01 g/L 0.09 mM
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