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Soil Extract Medium  

Directions 
General purpose freshwater medium suitable for axenic and xenic cultures. Modification of Bristol medium.
For 1 L Total
1. Prepare 1 L of Bristol Medium and thoroughly mix.
2. Discard 40 mL of the Bristol Medium.
3. Add 40 mL of previously prepared GR+ Medium [Note: The GR+ should be filtered to remove soil particles].
*  or  For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. Store at refrigerator temperature.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Bristol Medium960 mL  
2Soilwater: GR+ Medium40 mL of supernatant  

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