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Erdschreiber's Medium  

Directions 
Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].

For 3 L Total
1. To 3 L of pasteurized filtered seawater (30 ppt) aseptically add each of the sterile components in the order specified.
2. Vigorously swirl the contents of the flask to mix thoroughly.
3. Store at refrigerator temperature.
  
#ComponentAmountStock Solution
Concentration		Final Concentration
1Pasteurized Seawater3 L  
2P-IV Metal Solution36 mL/3 L  
3NaNO3
(autoclave before adding) (Fisher BP360-500)		10 mL/3 L0.7 M2.3 mM
4Na2HPO4·7H2O
(autoclave before adding) (Sigma S-9390)		10 mL/3 L0.02 M0.067 mM
5Soilwater: GR+ Medium150 mL/3 L  
6Vitamin B123 mL/3 L  
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