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DYIII Medium

Directions

Modification of Sandgren's recipe. Suitable for xenic cultures of freshwater chrysophytes.

For 1 L Total
1. To approximately 950 mL of dH₂O add each of the components in the order specified (except vitamins) while stirring continuously.
2. Adjust the pH to 6.7.
3. Bring the total volume to 1 L with dH₂O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
6. Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	MES (Sigma M-8250)	1.95 g/L		10 mM
2	MWC Metal Solution	10 mL/L
3	CaCl₂·2H₂O (Sigma C-3881)	1 mL/L	2 g/100 mL	0.14 mM
4	MgSO₄·7H₂O (Sigma 230391)	1 mL/L	7.4 g/100 mL	0.3 mM
5	Na₂glycerophosphate^.5H₂O (Sigma G 6501 )	1 mL/L	1 g/100 mL	0.03 mM
6	NaNO₃ (Fisher BP360-500)	1 mL/L	2 g/100 mL	0.24 mM
7	Na₂SiO₃·9H₂O (Sigma 307815)	1 mL/L	1.5 g/100 mL	0.05 mM
8	NH₄NO₃ (Fisher A676)	1 mL/L	1 g/100 mL	0.12 mM
9	KCl (Fisher P 217)	1 mL/L	1 g/100 mL	0.13 mM
10	Vitamin B₁₂	1 mL/L
11	Biotin Vitamin Solution	1 mL/L
12	Thiamine Vitamin Solution	1 mL/L

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