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Dasycladales Seawater Medium  

Directions 
For 3 L Total
1. Prepare 3 L of Pasteurized Seawater (30 ppt).
2. Add the remaining sterile media solutions to the flask in the order listed.
3. Store at refrigerator temperature.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Pasteurized Seawater3 L  
2Soil+Seawater Medium50 mL/3 L  
3DAS Macro Solution15 mL/3 L  
4Modified P-IV chelated Micronutrient Solution30 mL/3 L  
5DAS Vitamin Cocktail1.5 mL/3 L  

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