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Soilwater: BAR Medium  

Directions 
Suitable for some mixotrophic and most heterotrophic (colorless) euglenoids.

For 200 mL Total
1. Combine all components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
3. Refrigerate 24 hours or more and bring to room temperature before use.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Green House Soil5 mL/200 mL of dH20  
2Barley grains
(add after dH2O)
2 per 200 mL  
3CaCO3 (optional) (Fisher C 64)1 mg/200 mL of Seawater 0.05 mM

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