|
Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures, especially blue-greens and reds.
For 1 L Total
1. To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. When cooled add Vitamin B12.
*For agar medium add vitamin, mix, and dispense before agar solidifies.
5. Store at refrigerator temperature.
|
