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Modification of M.M. Allen's freshwater medium for blue-greens. Suitable for axenic and xenic cultures. The marine LB 1928 Spirulina platensis [Medium: ES-enriched seawater] can be adapted to Allen medium (D. James 1982, pers. Comm.).
For 1 L Total
1. To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Adjust pH to 7.8.
3. Bring total volume to 1 L with dH2O.
*For 1% agar medium: add 10 g of agar to the flask; do not mix.
4. Cover and autoclave medium.
5. Store at refrigerator temperature.
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