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BG-11(-N) Medium

Directions

For 1 L Total

Liquid media:
1. To approximately 900 mL of dH₂O add the first 8 components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH₂O.
3. Cover and autoclave medium.
4. Allow to cool then store at refrigerator temperature.

Agar media:
1. To approximately 400 mL of dH₂O add the first 8 components in the order specified while stirring continuously.
2. Bring total volume to 500 mL with dH₂O.
3. In a separate container add 15 g of agar to 500 mL of dH₂O (final 1.5% w/v).
4. Cover and autoclave both solutions.
5. In a water bath allow both solutions to cool to 45-50 °C.
6. Add sterile Sodium Thiosulfate to agar solution and mix well.
7. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
8. Allow to cool then store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	K₂HPO₄ (Sigma P 3786)	10 mL/L	0.8 g/200 mL	0.22 mM
2	MgSO₄·7H₂O (Sigma 230391)	10 mL/L	0.15 g/200 mL	0.03 mM
3	CaCl₂·2H₂O (Sigma C-3881)	10 mL/L	0.72 g/200 mL	0.24 mM
4	Citric Acid·H₂O (Fisher A 104)	10 mL/L	0.12 g/200 mL	0.012 mM
5	Ammonium Ferric Citrate	10 mL/L	0.12 g/200 mL	0.02 mM
6	Na₂EDTA·2H₂O (Sigma ED255)	10 mL/L	0.02 g/200 mL	0.002 mM
7	Na₂CO₃ (Baker 3604)	10 mL/L	0.4 g/200 mL	0.18 mM
8	BG-11 Trace Metals Solution	1 mL/L
9	Sodium Thiosulfate (agar media only,sterile)	1 mL/L	49.6 g/200 mL	1 µM

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