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Tap Medium  

Directions 
For 2 L Total
1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 2 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. Allow to cool and store at refrigerator temperature.
*For agar medium dispense before agar solidifies.

  
#ComponentAmountStock Solution
Concentration
Final Concentration
1Beijerinck's Solution100 mL/2 L  
2Phosphate Buffer Stock Solution17 mL/2 L  
3Hunter's Trace Stock Solution2 mL/2 L  
4Tris Acetate Stock Solution20 mL/2 L  

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