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2X Erdschreiber's Medium

Directions

Erdschreiber's Medium made with Supplemented Seawater instead of Pasteurized Seawater.

For 3 L Total
1. To 3 L of pasteurized supplemented seawater (60 ppt) aseptically add each of the sterile components in the order specified.
2. Vigorously swirl the contents of the flask to mix thoroughly.
3. Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Supplemented Seawater	3 L
2	P-IV Metal Solution	36 mL/3 L
3	NaNO₃ (Fisher BP360-500)	10 mL/3 L	0.7 M	2.3 mM
4	Na₂HPO₄·7H₂O (Sigma S-9390)	10 mL/3 L	0.02 M	0.067 mM
5	Soilwater: GR+ Medium	150 mL/3 L
6	Vitamin B₁₂	3 mL/3 L

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