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Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures.
For 1 L Total
1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
6. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
7. Store at refrigerator temperature.
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