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P-IV Metal Solution  

Directions 
For 1 L Total
Note final concentration listed is for the stock solution.
1.To approximately 950 mL of dH2O, add the nutrients in the order listed while stirring continuously.
Note: The Na2EDTA should be fully dissolved before adding other components.
2. Bring total volume to 1 L with dH2O.
3. Store at refrigerator temperature.
  
#ComponentAmountStock Solution
Concentration		Final Concentration
1Na2EDTA·2H2O (Sigma ED255)0.75 g/L 2 mM
2FeCl3·6H2O (Sigma F-1513)0.097 g/L 0.36 mM
3MnCl2·4H2O (Baker 2540)0.041 g/L 0.21 mM
4ZnCl2 (Sigma Z-0152)0.005 g/L 0.037 mM
5CoCl2·6H2O (Sigma C-3169)0.002 g/L 0.0084 mM
6Na2MoO4·2H2O (J.T. Baker 3764)0.004 g/L 0.018 mM
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