For some marine and brackish-water algae requiring salinities at 15 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/2 Soil+Seawater Medium contains seawater combined with distilled water 1:1.
For 200 mL Total
1. Combine all components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95°C in 15 minutes. Then increased just over 98°C for the 3 hour duration. Cooling occurs gradually at room temperature.
3. Refrigerate 24 hours or more and bring to room temperature before using.